Highly sensitive enhanced chemiluminescence kit for the detection of immobilized proteins (Western Blot) or immobilized nucleic acids (Southern and Northern Blot) labelled directly with horseradish peroxidase (HRP) or indirectly with HRP-labelled antibodies/streptavidin.

The substrate is readily prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a one-to-one ratio.
0.1 ml substrate is sufficient for one cm² membrane.
· High sensitivity, low picogram limit of detection
· Long light emission for 8 hours
· Working solution stable for at least three days - reproducible results, less waste
· Primary antibody dilution 1:500 -1:5000
· Secondary antibody dilution 1:20.000 – 1:100.000
· Detection can be done by film or CCD imaging equipment