SERVALight Eos CL HRP WB Substrate Kit
- Pack Sizes
- Certificates of Analysis (Lot.-no. - release date)
- PDF Documents
- Additional Information
- Related Products
- Product Links
Very highly sensitive enhanced chemiluminescence kit for the detection of immobilized proteins (Western Blot) or immobilized nucleic acids (Southern and Northern Blot) labelled directly with horseradish peroxidase (HRP) or indirectly with HRP-labelled antibodies/streptavidin.
The substrate is readily prepared by mixing component A (luminol/enhancer
solution) with component B (stabilized peroxide solution) in a one-to-one
ratio.
0.1 ml substrate is sufficient for one cm2 membrane.
·
Very high sensitivity, mid femtogram limit of detection
· Very long and
steady light emission for >24 hours
· Primary antibody dilution 1:1000
-1:15.000
· Working solution stable at least for three days - reproducible
results, less waste
· Secondary antibody dilution 1:25.000 – 1:150.000
·
Detection can be done by film or CCD imaging equipment
HS: 38229000
Storage Temperature: +2 °C to +8 °C