Ready-to-use substrate solution for chemiluminescence detection of membrane bound antigens or nucleic acid sequences directly with Horseradish Peroxidase (HRP) or indirectly with HRP-conjugated antibodies or Streptavidin labelled (1). Prior before use add 30 % hydrogen peroxide in a 1:1000 dilution (not provided) and use 100 µl/cm².

• High resolution and sensitivity

• Short exposure time

• Document your results on film or with a chemiluminescence applicable gel documentation system.

1. Thorpe, G.H.G. and Kricka, L. J. (1986) Methods Enzymol. 133, 331 – 353