The XTT Cell Proliferation Assay is a colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT is reduced to a highly coloured formazan dye by dehydrogenase enzymes in metabolically active cells. This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample. The formazan dye formed in the assay is soluble in aqueous solution and can be quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as N-methylphenazonium methyl sulfate (PMS) can significantly improve the efficiency of XTT reduction in cells.

• Easy to use - no need for additional reagents and washing procedures

• Rapid and sensitive – no solubilisation step, works with low cell concentrations

• Accurate – dye absorbance proportional to the number of cells/well


Content: 2x 25 ml XTT Reagent, 2x 0.5 ml Activation Reagent



HS: 38229000
Storage Temperature: -15 °C to -25 °C