SingleQuant Assay Kit


Single tube format assay kit for protein quantification. The assay bases on the precipitation of proteins as insoluble dye complexes with acidic, ethanolic amido black 10B solution (1,2). After precipitation the protein-dye complexes are spinned down. The pellet is washed and resolubilized. The thereby released dye amount is measured at 624 nm.

  • Precise, reproducible, reliable assay data
  • Completed in only 45 min.
  • No interference with detergents or reducing agents
  • Detection range starts as low as 2 µg protein.



References:
  1. Schaffner W., Weissmann C. (1973) Anal. Biochem. 65: 502-514.
  2. Popov N., Schmitt M., Schulzeck S., Matthies H. (1975) Acta Biol. Med. Ger. 34 (9): 1441-1446.
DANGER
Hazard Statements H225 - H314
Precautions P243 - P280
Reaction P301 +P330 +P331 - P302 +P352 - P304 +P340 - P305 +P351 +P338

HS: 38220000
Storage Temperature: +2 °C to +8 °C

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H225

Deutsch:

Flüssigkeit und Dampf leicht entzündbar.

English:

Highly flammable liquid and vapour.

Français:

Liquide et vapeurs très inflammables.

Italiano:

Liquido e vapori facilmente infiammabili.

Español:

Líquido y vapores muy inflamables.

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H314

Deutsch:

Verursacht schwere Verätzungen der Haut und schwere Augenschäden.

English:

Causes severe skin burns and eye damage.

Français:

Provoque des brûlures de la peau et des lésions oculaires graves.

Italiano:

Provoca gravi ustioni cutanee e gravi lesioni oculari.

Español:

Provoca quemaduras graves en la piel y lesiones oculares graves.

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P243

Deutsch:

Maßnahmen gegen elektrostatische Aufladungen treffen.

English:

Take precautionary measures against static discharge.

Français:

Prendre des mesures de précaution contre les décharges électrostatiques.

Italiano:

Prendere precauzioni contro le scariche elettrostatiche.

Español:

Tomar medidas de precaución contra descargas electrostáticas.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P301 +P330 +P331

Deutsch:

BEI VERSCHLUCKEN: Mund ausspülen. KEIN Erbrechen herbeiführen.

English:

IF SWALLOWED: rinse mouth. Do NOT induce vomiting.

Français:

EN CAS D’INGESTION: rincer la bouche. NE PAS faire vomir.

Italiano:

IN CASO DI INGESTIONE: sciacquare la bocca. NON provocare il vomito.

Español:

EN CASO DE INGESTIÓN: Enjuagarse la boca. NO provocar el vómito.

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P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

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P304 +P340

Deutsch:

BEI EINATMEN: An die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: transporter la victime à l’extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.