The Salt Active Nuclease completely degrades DNA and RNA in cell extracts and protein samples under high salt conditions. In the range of the activity optimum at 0.5 M NaCl, nucleic acids bound to proteins and protein complexes dissociate, whereby the endonuclease isolated from a marine bacterium also degrades DNA and RNA molecules that are inaccessible under low-salt conditions. The reaction takes place at a pH between 7 and 10 at low temperatures and in traditional buffer systems to protect the proteins.

• Non-specific endonuclease

• Optimum activity at high salt concentration (0.5 M NaCl)

• Active at low temperatures (20 % at 6 ºC)

• Broad pH range

• Easily inactivated by reducing agents


Supplied as solution in 25 mM Tris-HCl pH 7.5, 5 mM MgCl₂, 0.5 M NaCl, 0.01 % Tween 20, 50 % (v/v) glycerol.









pH optimum Salt optimum

pH 9 0.5 M NaCl

Unit definition: One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute at 37 ºC, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25 ºC), 5 mM MgCl₂, 500 mM NaCl.


HS: 38229000
Storage Temperature: -15 °C to -25 °C