GST-TAG Purification
Fusion proteins expressed from pGEX vectors contain a Glutathione S-Transferase (GST) moiety and can therefore be purified to near homogeneity by affinity chromatography of glutathione as a substrate to inactivate toxic small molecules via formation of mercapturic acid. Because the affinity of GST for its substrate is in the submilimolar range, immobilization of glutathione on an agarose matrix makes a highly efficient affinity chromatography resin.
SERVA’s
Glutathione Agarose Resin allows a rapid, mild and highly selective
purification of GST fusion proteins in a one step procedure. It can be used in
batch or column purifications. For column purification we offer empty columns
for small volumes of 50 – 100 µl and 200 – 250 µl or larger volumes of 0.5 – 2
ml and 2 – 6 ml.