Sample Preparation

Mammalian Total Protein Extraction Kit - Fast and Simple Isolation of Native Cytoplasmic, Membrane and Nuclear Proteins

  • Contains ready-to-use, optimized extraction buffer and protease inhibitor cocktail 
  • For protein isolation from cells and tissues
  • Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
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Mammalian Membrane Protein Extraction Kit - Fast and Simple Isolation of Native Membrane Proteins

  • Contains ready-to-use, optimized extraction buffers and protease inhibitor cocktail 
  • Simple and effcient isolation of membrane and cytoplasmic protein fractions from cells and tissues within 75 min
  • Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
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Mammalian Nuclear and Cytoplasmic Protein Extraction Kit - Fast and Simple Isolation of Native Nuclear and Cytoplasmic Proteins

  • Contains ready-to-use, optimized extraction buffers and protease inhibitor cocktail 
  • Simple and effcient isolation of nuclear and cytoplasmic protein fractions from cells and tissues within 80 min
  • Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
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Ni-Extrachel Agarose Resin - One-Step Purification without the Need of Pretreatment of Samples

  • Polychelator ligand covalently coupled to a highly crosslinked agarose resin
  • One-step purification without the need of pretreatment of samples for removal of nickel stripping agents
  • Higher resistance against EDTA, DTT, ethanol, etc. as resins of other vendors
  • Binding capacity: > 80 mg/ml gel
  • Suitable for batch, gravity and high pressure column purification.

 


Recombinant PNGase F - The Choice for Use in MALDI-Imaging due to Faster Kinetics and Greater Activity against Native Proteins

  • Mutant recombinant glycosidase from Flavobacterium meningosepticum and expressed and purified from E. coli
  • Specifically removes N-linked oligosaccharides from glycoproteins
  • Does not need a denaturing step
  • Works on native glycoproteins and serum glycoproteins in minutes at room temperature
  • Digestion leads to a more complete glycan release as compared to other commercially-available enzymes
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Salt Active Nuclease - Only Endonuclease with an Optimum Activity in 0.5 M NaCl

  • Digests as well protein-bound DNA due to optimum activity in 0.5 M NaCl 
  • Active at low temperatures and high pH range
  • Easily inactivated by denaturing reagents
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Recombinant Proteinase K - Suitable for Application in Sensitive PCR Reactions

  • Endopeptidase with very high specific activity and broad spectrum of action
  • Digestion of proteins in nucleic acids preparations without compromising integrity of isolated DNA or RNA
  • Improves cloning efficiency of PCR products
  • Free of endonucleases, exonucleases and ribonucleases
  • NGS grade with 2.5 higher solubility and higher specific activity

 

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BlueZol - Ready-to-Use Reagent for the Rapid Isolation of RNA, DNA and Proteins from a Single Sample

  • For the rapid isolation of total RNA from cells and tissues of human, animal, plant, yeast or bacterial origin
  • Due to highly effective RNase inhibitory properties the integrity of the RNA during lysis is fully protected
  • Suitable for the simultaneous isolation of RNA, DNA and protein from one sample
  • Purified RNA is ideal for any downstream applications such as RT-PCR, in vitro translation, Northern Blotting, RNase protection assays or dot blot hybridization
  • Purified DNA can be used for PCR and Southern Blotting and the proteins for Western Blotting
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CTAB DNA Extraction Buffer for DNA Isolation from Plants

Ready-to-use buffer with the non-ionic detergent cetyltrimethylammonium-bromide (CTAB) for removal of polysaccharides contamination during isolation of DNA from plants. By adjustment of salt concentration in lysates containing CTAB, polysaccharides and DNA can be differentially precipitated.

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Xpress Micro Dialyzer - Rapid Dialysis of Sample Volumes of 10 - 300 µl

  • High yield - up to 98 % sample recovery
  • Easy sample loading and retrieval with standard laboratory micropipettes
  • Efficient and fast desalting of nucleic acids and proteins in ca. 30 min
  • Flexible – scalable from 1 to 96 samples, dialysis in micro tubes/microtiter plates or in beakers
  • Automation compatible – plate format conforms to the SBS Microplate Standard
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Xpress Mini Dialyzer - Rapid Dialysis of Sample Volumes of 150 µl - 1 ml

  • High yield - up to 98 % sample recovery
  • Easy sample loading and retrieval with standard laboratory micropipettes
  • Efficient and fast desalting of nucleic acids and proteins in ca. 30 min
  • Flexible – scalable from 1 to 48 samples, dialysis in micro tubes/microtiter plates or in beakers
  • Automation compatible – plate format conforms to the SBS Microplate Standard
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ReadyLyzer - Ready-to-use Dialysis System for Quick and Efficient Dialysis and Buffer Exchange

  • Easily opened and closed with a screw cap
  • No struggling with closures and leaking knots or risk of puncturing the membrane
  • Delivered with a flotation ring for improved buoyancy and vertical orientation
  • Available volume sizes: 10 - 250 µl, 50 – 800 µl, 0.1 – 3 ml, 10 ml, 20 ml
  • High recovery 98 %
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SERVA BlueMarine™ 200

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Product of the Month July:
SERVA BlueMarine™ 200

For Electrophoresis of Nucleic Acids on Agarose Gels

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