Sample Preparation
Mammalian Total Protein Extraction Kit - Fast and Simple Isolation of Native Cytoplasmic, Membrane and Nuclear Proteins
- Contains ready-to-use, optimized extraction buffer and protease inhibitor cocktail
- For protein isolation from cells and tissues
- Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
Mammalian Membrane Protein Extraction Kit - Fast and Simple Isolation of Native Membrane Proteins
- Contains ready-to-use, optimized extraction buffers and protease inhibitor cocktail
- Simple and effcient isolation of membrane and cytoplasmic protein fractions from cells and tissues within 75 min
- Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
Mammalian Nuclear and Cytoplasmic Protein Extraction Kit - Fast and Simple Isolation of Native Nuclear and Cytoplasmic Proteins
- Contains ready-to-use, optimized extraction buffers and protease inhibitor cocktail
- Simple and effcient isolation of nuclear and cytoplasmic protein fractions from cells and tissues within 80 min
- Extracted proteins are suitable for SDS-PAGE, Western Blot, ELISA and other functional assays
PNGase F - The Choice for Use in MALDI-Imaging due to Faster Kinetics and Greater Activity against Native Proteins
- Mutant recombinant glycosidase from Flavobacterium meningosepticum and expressed and purified from E. coli
- Specifically removes N-linked oligosaccharides from glycoproteins
- Does not need a denaturing step
- Works on native glycoproteins and serum glycoproteins in minutes at room temperature
- Digestion leads to a more complete glycan release as compared to other commercially-available enzymes
Endo F3 - Especially designed and tested for MALDI-Imaging and HPLC/UPLC
- For the structural characterisation of nuclear fucosylated glycans in tissues
- Used alone or in combination with PNGase F
- Contains a His-tag for easy removal by affinity chromatography
- No refrigerated transport necessary, as lyophilised
Endo S - For Glycoengineering Strategies for the Development of Therapeutic IgG Antibodies
- Unique accuracy in the cleavage of N-linked glycans from the chitobiose core of the heavy chain of native IgG molecules
- Specifically designed and tested for mass spectrometry and HPLC/UPLC
- Excellent performance - produces clean peaks with very high yield in HPLC
- Contains a His-tag for easy removal by affinity chromatography
- No refrigerated transport necessary as lyophilised
Sialidase - For Complete Removal of Sialic Acids from Glycoconjugates
- Recombinant exoglycosidase from Arthrobacter ureafaciens
- Cleaves α2,3-, α2,6- and α2,8- bound sialic acids
- Specially developed and tested for mass spectrometry and HPLC/UPLC
- Contains a His-tag for easy removal by affinity chromatography
- No refrigerated transport necessary, as lyophilised
Zymolyase® - For production of proto- or spheroplasts of various strains of yeast cells
- Isolated from Arthrobacter luteus
- Strong lytic activity for the cell walls of a variety of yeast strains
- Essential enzyme activity is β-1,3-glucanlaminaripentaohydrolase
Salt Active Nuclease - Only Endonuclease with an Optimum Activity in 0.5 M NaCl
- Digests as well protein-bound DNA due to optimum activity in 0.5 M NaCl
- Active at low temperatures and high pH range
- Easily inactivated by denaturing reagents
Recombinant Proteinase K - Suitable for Application in Sensitive PCR Reactions
- Endopeptidase with very high specific activity and broad spectrum of action
- Digestion of proteins in nucleic acids preparations without compromising integrity of isolated DNA or RNA
- Improves cloning efficiency of PCR products
- Free of endonucleases, exonucleases and ribonucleases
- NGS grade with 2.5 higher solubility and higher specific activity
BlueZol - Ready-to-Use Reagent for the Rapid Isolation of RNA, DNA and Proteins from a Single Sample
- For the rapid isolation of total RNA from cells and tissues of human, animal, plant, yeast or bacterial origin
- Suitable for the simultaneous isolation of RNA, DNA and protein from one sample
- RNA is suitable for any downstream applications such as RT-PCR, in vitro translation, Northern Blotting, RNase protection assays or dot blot hybridization
- DNA can be used for PCR and Southern Blotting and the proteins for Western Blotting
Ni-Extrachel Agarose Resin - One-Step Purification without the Need of Pretreatment of Samples
- Polychelator ligand covalently coupled to a highly crosslinked agarose resin
- One-step purification without the need of pretreatment of samples for removal of nickel stripping agents
- Higher resistance against EDTA, DTT, ethanol, etc. as resins of other vendors
- Binding capacity: > 80 mg/ml gel
- Suitable for batch, gravity and high pressure column purification.
Xpress Micro Dialyzer - Rapid Dialysis of Sample Volumes of 10 - 300 µl
- High yield - up to 98 % sample recovery
- Easy sample loading and retrieval with standard laboratory micropipettes
- Efficient and fast desalting of nucleic acids and proteins in ca. 30 min
- Flexible – scalable from 1 to 96 samples, dialysis in micro tubes/microtiter plates or in beakers
- Automation compatible – plate format conforms to the SBS Microplate Standard
Xpress Mini Dialyzer - Rapid Dialysis of Sample Volumes of 150 µl - 1 ml
- High yield - up to 98 % sample recovery
- Easy sample loading and retrieval with standard laboratory micropipettes
- Efficient and fast desalting of nucleic acids and proteins in ca. 30 min
- Flexible – scalable from 1 to 48 samples, dialysis in micro tubes/microtiter plates or in beakers
- Automation compatible – plate format conforms to the SBS Microplate Standard
ReadyLyzer - Ready-to-use Dialysis System for Quick and Efficient Dialysis and Buffer Exchange
- Easily opened and closed with a screw cap
- No struggling with closures and leaking knots or risk of puncturing the membrane
- Delivered with a flotation ring for improved buoyancy and vertical orientation
- Available volume sizes: 10 - 250 µl, 50 – 800 µl, 0.1 – 3 ml, 10 ml, 20 ml
- High recovery 98 %
