Dialysis

Dialysis is a process where substances in solution are separated by their differences in molecular weight. The dialysis tubings are semipermeable, made of regenerated cellulose or cellulose ester having a gel-like structure. The driving force is the concentration difference between 2 solutions on opposite sides of the membrane. Since the porous membrane selectively allows smaller solutes to pass while retaining larger species, dialysis can effectively be used as a separation process based on size rejection.

Since the dialysis membrane consists of a spongy matrix of crosslinked polymers, the pore rating referred to as Molecular Weight Cut Off (MWCO), is an indirect measure of the retention performance. The membrane MWCO is determined as the solute size that is retained by at least 90 %. However, since a solute’s permeability is also dependent upon molecular shape, degree of hydration, ionic charge and polarity, it is recommended to select a MWCO that is half the size of the MW of the species to be retained and/or twice the size of the MW of the species intended to pass through.

For many years, dialysis has been successfully used as a routine method for desalting and concentration of complex biomolecules in solution. In addition, macromolecules, differing substantially in molecular weight can be separated.

Applications

  • Desalting of protein solutions, e.g. for further purification by ion exchange chromatography
  • Concentration of protein solutions by embedding the filled tubing in dry PEG 20000 (cat. no. 33138) e.g. before further separation by gel permeation chromatography
  • Separation of high molecular weight proteins from low molecular weight peptides and other substances
  • Buffer exchange with solutions of proteins or other macromolecules
  • Desalting of metallo colloids


News

SERVA Catalog
2023

Electrophoresis by SERVA - Catalog

Watch the SERVA Video!

Certificates of Analysis
now online!

Top
Close window
SERVA Catalog
2023

In detail you will find the following new products in catalog 2022:

New glycosidases such as Endo F3, Endo S and Sialidase
Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
SERVA Catalog 2023.pdf
Close window
Electrophoresis by SERVA - Catalog

• Isoelectric Focusing

• SDS PAGE

• 2D Electrophoresis

• Native Electrophoresis

• Western Blotting

• Nucleic Acid Electrophoresis

• Equipment

SERVA Electrophoresis Catalog (PDF)
Close window
Watch the SERVA Video!

We are SERVA! Watch to see where we are located and how we are serving scientists worldwide.

SERVA Video

Close window
Certificates of Analysis
now online!

How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

Close window
SERVA - now on YouTube

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.