SDS PAGE

The mostly used electrophoresis method for protein separation and analysis is the SDS polyacrylamide gel electrophoresis (SDS PAGE). The sample and the running buffer contain the detergent sodium dodecylsulfate (SDS).

The proteins and the SDS form micelles which migration speed in the gel matrix is relative to the molecular weight of the proteins. Due to this, the SDS PAGE allows separation of proteins according to the molecular weight. 

PRIME-Pakete 2017

Furthermore, all proteins have a negative net charge and migrate towards the anode (+). The proteins are separated in a polyacrylamide gel matrix.

The separation performance and resolution of the gel can be varied by the acrylamide concentration and the running buffer.

After separation, the protein bands are detected by staining either with colorimetric (Coomassie®, silver) or fluorescent dyes.

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SERVA Market Square: March - June 2020

Product of the Month April:
SERVAColor Blot Solutions

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SERVA Market Square: March - June 2020

• Vertical Protein Electrophoresis
• Horizontal Protein Electrophoresis
• Western Blotting
• Fluorescent  Dyes
• DNA/RNA Electrophoresis
• Fluorescence Labelling
• Cell based Assays
• Enzymes
• Detergents
• Protease- and Phosphatase Inhibitors

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Product of the Month April:
SERVAColor Blot Solutions

SERVAColor Blot Solutions - for detection of alkaline phosphatase or horseradish peroxidase on Western blot membranes

→ This way to the Special Offer

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