RNA & cDNA
The products for RNA & cDNA are divided into the following subcategories:
- RNA Synthesis
- cDNA Synthesis
- RNases
- RNA Protection
If you have any questions about the products, please feel free to contact us at info@serva.de. Since not all products are listed on our website yet, you can also send order inquiries to info@serva.de or directly to order@serva.de.
For more information and additional products, visit NZYTech’s website. We offer the same product prices and deliver directly from Germany!
RNA Synthesis
NZYtech offers convenient kits for the in vitro transcription of DNA into RNA using a T7 RNA polymerase that is highly specific for T7 phage promoters. The enzyme is also provided separately for your routine protocols.
cDNA Synthesis
Reverse Transcriptases
NZY M-MuLV and NZY Reverse Transcriptases lack 3’-5’ exonuclease activity and have no intrinsic RNase H activity. NZY Reverse Transcriptase is a thermostable (50-55 °C) and sensitive enzyme with fast synthesis capacity (30 min reaction). Supreme NZYReverse Transcriptase was recently developed to increase inhibitor resistance, range and thermostability.
cDNA Kits
NZYtech offers convenient, reliable and cost-effective kits to generate high quality cDNA for different downstream applications, such as standard PCR, cDNA library construction, or two-step RT-PCR assays. cDNA kits contain all the components required to synthesize first-strand cDNA (except the template RNA), at optimal conditions. A mixture of NZY Ribonuclease Inhibitor and one of the NZYtech’s reverse transcriptases is included in all kits. NZY First-Strand cDNA Synthesis kits provide more specific synthesis of cDNA, while NZY M-MuLV First-Strand Synthesis kits are a cost-effective alternative, also providing high yields of full-length cDNA at low reaction temperatures.
RNA Primers
Good to know:
Oligo(dT)18 primer: hybridizes to the poly(A) tail found at the 3´- end of most eukaryotic mRNAs; frequently used when cDNA is used for cloning, cDNA library construction and qPCR
Random hexamers: priming ssDNA or RNA for extension by DNA polymerase or Reverse transcriptases; during cDNA synthesis, random hexamer will perform random priming throughout the entire length of the RNA to generate a cDNA pool containing various lengths of cDNA
RNA Protection
NZYtech Ribonuclease Inhibitors are active againts RNases (E. C. 3.1) of the pancreatic type (RNase A, B and C) and are useful in applications where eukaryotic RNase contamination is a potential problem. For reactions that do not tolerate higher levels of DTT, please use the oxidation-resistant NZY Ribonuclease Inhibitor (no DTT). NZYtech offers a powerful trio of cleaners targeting DNA, RNA, Mycoplasma, and nucleases, ensuring thorough decontamination for optimal laboratory operations
