Product of the Month August

Proteinase K

Proteinase K from Tritirachium album expressed recombinantly  in Pichia pastoris

Proteinase K is a subtilisin-like serine protease with a broad substrate specificity. The enzyme degrades both terminal (exopeptidase activity) and internal (endopeptidase activity) peptide bonds.
It is widely used for digestion of proteins, including DNases and RNases during nucleic acid preparations without compromising the integrity of the isolated DNA or RNA.
During production of the recombinant enzyme almost all nucleic acids are removed. Therefore, it is especially suitable for PCR reactions.

Application
Inactivation and degradation of nucleases during isolation of nucleic acids from tissues, pro- or eukaryotic cells.

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Features

  • Highly purified lyophilisate
  • Specific activity: ca. 30 U/mg protein
  • Broad substrate specificity
  • DNase/RNaseactivity not detectable
  • DNA content: ≤10 pg/mg enzyme
  • Active in the pH range:4.0 -12.0
    (
    optimum: 7.5 -8.5)
 
  • Active in the temperature range:20 °C to
    65 °C(maximum activity at50 °C to 65 °C)
  • Inhibition by the following serine protease inhibitors: AEBSF, PMSF and DFP
  • Unaffected by metal-chelating agents and sulfhydryl inhibitors

Ordering Information:

Product
 Cat. No.
Size
Price
Special Price*
Proteinase K recombinant min. 30 mAnson-U/mg
molecular biology grade
33756.02
33756.03
100 mg
500 mg
    65.00 €
  211.00 €
    52.00 €
  168.80 €

*The special prices are valid until August 31st, 2020
 
Please Note:
The "Product of the Month" promotion is for all SERVA customers within the EU and cannot be combined with other discounts, promotions etc.
 

 Further Qualities of  Proteinase K

 

Reagents for SARS-CoV2 Detection

  • Ni-NTA Agarose Resins

  • Reagents for Molecular Biology

  • Reagents for Biochemistry

  • Reagents for  ELISA


Find here the list of SERVA Reagents for SARS-CoV2 Detection (PDF)

 


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Problems with 2D electrophoresis?

Here you will find helpful advices:

SERVA Webinar:
Sample Preparation for 2D Electrophoresis

If you have any further questions, please contact the SERVA team by phone (+49 6221 138 40-44) or by e-mail (Tech.Service@serva.de). 

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Promotions

SERVA Market Square: July - September 2020

Product of the Month August:
Proteinase K

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• Western Blotting
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Product of the Month August:
Proteinase K

For Effective Inactivation of Nucleases during Nucleic Adic Isolation

→ This way to the Special Offer

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