Product of the Month November

Proteinase K, NGS Grade

Highest Quality for Effective Inactivation of Nucleases during Nucleic Acid Isolation

Proteinase K from Tritirachium album expressed recombinantly in Pichia pastoris is a subtilisin-like serine protease with a broad substrate specificity. The enzyme degrades both terminal (exopeptidase activity) and internal (endopep-tidase activity) peptide bonds.
The enzyme is active under wide range of reaction conditions, including elevated temperatures and pre-sence of SDS. This recombinant enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.
An extra purification step during production results in significantly increased solubility (2.5-fold), increased specific activity, and decreased DNA content.
Therefore, it is especially suitable for methods demanding highest quality like Next Generation Sequencing (NGS).

Kachel POM Nov 19.png

 Features

  • Integrity of the isolated nucleic acids remains intact
  • Contains noendo-, exo-and ribonucleases
  • Solubility in water: ≥ 50 mg/ml
  • DNA content: ≤ 0,1 pg/mg enzyme
  • Serine protease
  • Increased stability by Ca2+(1 -6 mM)
  • Activated by 0.2 -1 % (w/v) SDS, 4 M urea
 
  • Active in the pH range: 4.0 -12.0
    (optimum: 7.5 -8.5)
  • Active in the temperature range: 20 °C to 65 °C (maximum activity at 50 °C to 65 °C)
  • Inhibition by the following serine protease inhibitors: AEBSF, PMSF and DFP
  • Unaffected by metal-chelating agents and sulfhydryl inhibitors

 Ordering Information:

Product
 Cat. No.
Size
Pice
Special Price*
Proteinase K recombinant,
min. 35 mAnson-U/mg, NGS Grade
 33757.01
33757.02
33757.03
    25 mg
  100 mg
5000 mg
   40.00 €
107.00 €
497.00 €
   32.00 €
  85.60 €
383.20 €

*The special prices are valid until November 3 th, 2019 
Please Note:
The "Product of the Month" promotion is for all SERVA customers within the EU and cannot be combined with other discounts, promotions etc..
 

Here you will find further sample preparation products, e.g.

Sample-Prep_1.png

SERVA@YouTube - Sample Preparation

Problems with 2D electrophoresis?

Find the links to the webinars with advices for sample preparation below:

YouTube.png


If you have any further questions, please contact the SERVA team by phone (+49 6221 138 40-44) or by e-mail (Tech.Service@serva.de). 

Promotions

Late Year Promotion until 31-12-2019
Enzymes & Reagents for MassSpec

Market Square
September - December 2019

Product of the Month November
Proteinase K, NGS Grade

Top
Close window
Late Year Promotion until 31-12-2019
Enzymes & Reagents for MassSpec

Enhzymes and Reagents for Mass Spectrometry

→ This way to the Special Offer

Close window
Market Square
September - December 2019

• Protein Electrophoresis - Reagents and Equipment
• Western Blotting
• Mini Vertical 2D Electrophoresis
• DNA/RNA-Elektrophoresis
• Micro- and Molecular Biology
• Dextrans & PEG, PVP
• Antibiotics
• Apoptosis
• Reagentis for Microskopy

This way to the new Market Square

Close window
Product of the Month November
Proteinase K, NGS Grade

Highest Quality for Effective Inactivation of Nucleases during Nucleic Acid Isolation

 → This way to the Special Offer

SERVA InfoMail - Get the latest information

Close window

When subscribing to »SERVA InfoMail« you will receive information about the latest product release, special promotions, new jobs at SERVA and more. This service is, of course, free of charge.
You may remove your name any time from the list of recipients.

Quick Order

Close window

Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.