ASB-16

Synthetic detergent with a C16 alkyl chain. Useful for solubilizing proteins for 2D-analysis. ASB-16 shows better protein solubilization properties than CHAPS and in some cases than ASB-14, resulting in improved detection of membrane proteins by 2D-electrophoresis.

Synonym: Amidosulfobetaine-16; 3-[N,N-Dimethyl-N-(3-palmitamidopropyl)-ammonio]-propane-
1-sulfonate

CAS registry number: [52562-29-5]

Molecular formula: C24H50N2O4S

Relative molecular mass: 462.7

Classification: Zwitterionic surfactant

Critical micellar concentration (CMC): 8 mM


Bibliography

Chevallet, M. et al. (1998) New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis. Electrophoresis 19, 1901-9.

Herbert, B. (1999) Advances in protein solbilization for two-dimensional electrophoresis. Electrophoresis 20, 660-3.

Flatman, P.W. (2005) Activation of ferret erythrocyte Na+-K+-2Cl- cotransport by deoxygenation. J. Physiol. 563, 421-31.

Domingues, C.C. et al. (2008) Solubilization of human erythrocyte membranes by ASB detergents. Braz. J. Med. Biol. Res. 41, 758-64.

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