Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.

For IEF, SERVA offers a comprehensive product line:

• SERVALYT™ Carrier Ampholytes
• SERVALYT™ PRECOTES/PreNets™ Precast Gels & Kits
• FocusGels Precast Horizontal Gels
• SERVAGel™ IEF Vertical Gels
• Protein Standards
• Buffers and Accessories
• Equipment