Agarose Media

The electrophoretic behavior of DNA and RNA in gels is effected by their size and shape. Both RNA and DNA can exist as single and double stranded molecules and the single stranded forms have significant secondary and tertiary structure. Therefore single stranded nucleic acids are best separated as denatured structures. This is most often an issue for ssDNA after sequencing reactions, and RNA which can be denatured by urea, methylmercuric hydroxide, formamide, formaldehyde and a number of other agents.

Agarose is a highly purified naturally occurring polysaccharide. Preparation of agarose gels involves simply dissolving the powdered agarose in buffer by heating. The agarose gels upon cooling. Like acrylamide, the pore size of an agarose gel is inversely dependent on the agarose concentration. The pores in agarose gels are generally much larger than those in acrylamide gels and are widely used in separation of nucleic acids. Low molecular weight nucleic acids and oligonucleotides, however, are usually separated by PAGE, due to smaller pore size of the gel matrix.

Many types of different agarose qualities optimized for specific applications are available, e.g.:

  • Agarose SERVA Wide Range (cat no. 11406) or Agarose for DNA Electrophoresis (cat. no. 11404) for routine DNA electrophoresis
  • Agarose SERVA FastSolve Tabletten (cat. no. 11407) – fast dissolved, wide separation ranges from
    100 bp - ≥30 kb, packed in a convenient blister pack preventing clumping
  • Agarose SERVA 3:1 (cat. no. 11385) and Agarose for PCR (cat. no. 11383) for high resolution separation of small (10 bp - 1000 bp) DNA, RNA and PCR fragments
  • Agaroses with low melting temperature like Agarose SERVA Low Melting (cat. no. 11408) for the easy recovery of DNA fragments from agarose




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SERVA Catalog
2020

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Certificates of Analysis
now online!

Western Blotting -
New Brochure!

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New !

SERVA Catalog
2020

In detail you will find the following new products in catalog 2020:

  • • SERVA TAE Clear G Agarose tablets for practical nucleic acid electrophoresis
  • • Novel enzymes such as recombinant PNGases F as lyophilized powder
  • • High-performance protein A/G magnetic agarose beads
  • • New buffers for molecular and cell biology
  • • SERVA BluePower® power supplies running from 300 Volt to 6000 Volt and from 150 mA to 2 A for gel electrophoresis and blotting
  • • Electrophoresis gel and blot automation: SERVA BlueStain is an innovative device for the treatment of automated gel and blot staining
SERVA Catalog 2020.pdf
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Certificates of Analysis
now online!

How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

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Western Blotting -
New Brochure!
  • • Molecular Weight Standards
  • • SDS PAGE for Western Blotting
  • • Transfer Membranes and Buffers
  • • Blocking Reagents
  • • Detection Systems
  • • Instruments for Western Blotting
SERVA Brochures: Western Blotting V1806 (pdf)
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