Agarose Media

The electrophoretic behavior of DNA and RNA in gels is effected by their size and shape. Both RNA and DNA can exist as single and double stranded molecules and the single stranded forms have significant secondary and tertiary structure. Therefore single stranded nucleic acids are best separated as denatured structures. This is most often an issue for ssDNA after sequencing reactions, and RNA which can be denatured by urea, methylmercuric hydroxide, formamide, formaldehyde and a number of other agents.

Agarose is a highly purified naturally occurring polysaccharide. Preparation of agarose gels involves simply dissolving the powdered agarose in buffer by heating. The agarose gels upon cooling. Like acrylamide, the pore size of an agarose gel is inversely dependent on the agarose concentration. The pores in agarose gels are generally much larger than those in acrylamide gels and are widely used in separation of nucleic acids. Low molecular weight nucleic acids and oligonucleotides, however, are usually separated by PAGE, due to smaller pore size of the gel matrix.

Many types of different agarose qualities optimized for specific applications are available, e.g.:

  • Agarose SERVA Wide Range (cat no. 11406) or Agarose for DNA Electrophoresis (cat. no. 11404) for routine DNA electrophoresis
  • Agarose SERVA FastSolve Tabletten (cat. no. 11407) – fast dissolved, wide separation ranges from
    100 bp - ≥30 kb, packed in a convenient blister pack preventing clumping
  • Agarose SERVA 3:1 (cat. no. 11385) and Agarose for PCR (cat. no. 11383) for high resolution separation of small (10 bp - 1000 bp) DNA, RNA and PCR fragments
  • Agaroses with low melting temperature like Agarose SERVA Low Melting (cat. no. 11408) for the easy recovery of DNA fragments from agarose




News

Electrophoresis by SERVA - Catalog 2022

SERVA Catalog
2022

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Certificates of Analysis
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Electrophoresis by SERVA - Catalog 2022

• Isoelectric Focusing

• SDS PAGE

• 2D Electrophoresis

• Native Electrophoresis

• Western Blotting

• Nucleic Acid Electrophoresis

• Equipment

SERVA Electrophoresis Catalog (PDF)
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SERVA Catalog
2022

In detail you will find the following new products in catalog 2022:

New glycosidases such as Endo F3, Endo S and Sialidase
Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"

 

SERVA Catalog 2022.pdf
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Certificates of Analysis
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How to find the required certificate of analysis for your product:
In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02.
Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow.
Now select the lot number from the list and download the PDF with a click. Done.

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Using the Quick Order field, you can add a product to your shopping cart with just one click. All you have to do: enter the Cat.No. as shown in our catalog in the format xxxxx.yy and click the Go!-Button.