FocusGels are available with different pH gradients, with or without sample slots. FocusGels are 0.65 mm thin polyacrylamide gels with T = 5 % gel concentration and C = 3 % crosslinking, which have a matrix optimized for IEF.
Catalysts as well as other acrylamide monomers and other non-toxic compounds have been removed from the matrix. Therefore, FocusGels are not toxic. The gels contain a special carrier ampholyte cocktail to achieve an optimal pH gradient. No electrode solutions and electrode stripes are used; the electrodes are placed directly on the gel surface. FocusGels 24S contain 24 pre-polymerized sample wells for easy application of up to 25 µl protein solution close to the anodal or cathodal side. The other FocusGel types do not contain sample wells. Here samples can be applied employing various sample applicators.