Protein Extraction Kits

RIPA buffer is a very effective buffer for lysis of cultured mammalians cells. It enables protein extraction from cytoplasmic, membrane and nuclear proteins, suitable for many applications like Western Blotting, protein purification and protein assays.
Since the buffer will disrupt protein-protein interactions, applications like immunoprecipitation and pull-down assays may be disturbed.

If for determination of protein activity and other functional assays integrity of protein structure is needed, SERVA's Mammalian Protein Extraction Kits allow the fast and easy preparation of total protein or cytoplasmic, membrane and nuclear protein fractions from cells or tissues.

  • Mild but efficient lysis with high protein yield
  • Performance is optimized and validated for several cell lines and tissues
  • No need for mechanical disruption
  • Reagents are compatible with standard protein quantification assays like BCA Protein Assay
  • Extracted proteins are directly applicable in downstream applications like Western Blot, ELISA, EMSA

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    SERVA Catalog

    In detail you will find the following new products in catalog 2021:

    • • New enzymes like dsDNase for highly specific degradation of doubled-stranded DNA
    • • Zymolyase® to produce yeast protoplast or spheroplast
    • • Tergitol™ as an alternative to Triton X-100
    • • Easy Peel 2D HPE™ gels for blotting of horizontal 2D gels
    • • New protein standards, e.g. SERVA Unstained Protein Standard 6.5 - 97 kDa
    • • BlueVertical™ PRiME™ Blot Modul for SERVA’s BV-104 electrophoresis tank
    • • IPG tray with electrode lid for 1st dimension protein separation on 7 cm to 24 cm IPG strips
    • • Special Pharma Edition of SERVA BlueStain automated gel staining device


    SERVA Catalog 2021 (pdf)
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    SERVA Brochures: Western Blotting V2110 (pdf)
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