Protein Extraction Kits

RIPA buffer is a very effective buffer for lysis of cultured mammalians cells. It enables protein extraction from cytoplasmic, membrane and nuclear proteins, suitable for many applications like Western Blotting, protein purification and protein assays.
Since the buffer will disrupt protein-protein interactions, applications like immunoprecipitation and pull-down assays may be disturbed.

If for determination of protein activity and other functional assays integrity of protein structure is needed, SERVA's Mammalian Protein Extraction Kits allow the fast and easy preparation of total protein or cytoplasmic, membrane and nuclear protein fractions from cells or tissues.

  • Mild but efficient lysis with high protein yield
  • Performance is optimized and validated for several cell lines and tissues
  • No need for mechanical disruption
  • Reagents are compatible with standard protein quantification assays like BCA Protein Assay
  • Extracted proteins are directly applicable in downstream applications like Western Blot, ELISA, EMSA



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    SERVA Catalog
    2023

    In detail you will find the following new products in catalog 2022:

    New glycosidases such as Endo F3, Endo S and Sialidase
    Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
    New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
    BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
    he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
    Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
    SERVA Catalog 2023.pdf
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    Electrophoresis by SERVA - Catalog

    • Isoelectric Focusing

    • SDS PAGE

    • 2D Electrophoresis

    • Native Electrophoresis

    • Western Blotting

    • Nucleic Acid Electrophoresis

    • Equipment

    SERVA Electrophoresis Catalog (PDF)
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