Tween® 20pure, Ph. Eur., USP

(Polysorbate 20, Polyoxyethylene sorbitan monolaureate)
Mr ca. 1200CAS [9005-64-5]

Tween® 20, belonging to the family of polysorbates, is a non-ionic surfactant, which effectively prevents non-specific reactions between antibodies, antigens and other molecules (1, 2). The detergent is used in sample preparation and signal transduction research for solubilisation of membrane components (3) and in density centrifugation of viruses (4). Tween® 20 serves as an emulsifier for the preparation of stable oil-in-water emulsions.

Used as a component of:
  • Wash buffer and blocking buffer in Western blot analyses
  • Blocking buffer for immunohistochemistry
  • Wash buffer for ELISA (Enzyme-linked Immunosorbent Assay)
  • Reaction mixture for PCR (polymerase chain reaction)

    n ca. 20, CMC (25 °C) 0.059 mM, HLB 16.7

    Free dioxane
    Free ethylene oxide
    Heavy metals
    Non animal origin
    max. 1 ppm
    max. 1 ppm
    max. 10 ppm
    Tween 20 = registered trademark of CRODA International PLC

    1. Thean, E.T. & Toh, B.H. (1989) Anal. Biochem. 177, 256-8
    2. Tovey, E.R. et al. (1989) Electrophoresis 10, 243-9
    3. Lund, S. et al. (1989) J. Biol. Chem. 264, 4907-15
    4. Boeye, A. & DeRees, A. (1989) Arch. Virol. 107, 77-84

    EINECS: 500-018-3WGK: 1LHS: 34024200
    Storage Temperature: +15 °C to +30 °C

  • Cat.No.
    500 g
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    5 kg
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    Certificates of Analysis (Lot.-no. - release date)

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