2,2'-Azinobis(3-ethylbenzthiazoline-6-sulfonic acid)·2NH4-saltcryst. analytical grade
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C18H16N4O6S4·2NH4 • Mr 548.69 • CAS [30931-67-0]
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Azinobis(3-ethylbenzthiazoline-6-sulfonic acid)·2NH4-salt, abbreviated ABTS, is a chromogen for peroxidase in enzyme-linked immunoassay (ELISA) (1). ABTS is a substrate and for laccase and angiotensin I-converting enzyme assay (2). A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1 % sodium dodecyl sulfate (SDS).
TLC: |
one spot |
References:
- Groome, N.P. (1980) J. Clin. Chem. Clin. Biochem. 18, 345-9
- Shin, T. et al. (1987) Anal. Biochem. 166, 380-8
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WARNING |
Hazard Statements | H315 - H319 - H335 |
Precautions | P280 |
Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P332 +P313 - P337 +P313 |
EINECS: 250-396-6 • WGK: 1 • HS: 29342080
Storage Temperature: +15 °C to +30 °C