3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonateresearch grade
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C32H58N2O7S • Mr 614.9 • CAS [75621-03-3]
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3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate or CHAPS is a mild, zwitterionic sulfobetaine detergent, which is non-denaturing to membrane proteins, can solubilize proteins, and disaggregate protein-protein interactions (1 - 5).
Due to its zwitterionic nature, CHAPS does not exhibit a net charge between pH 2 to 12, making it also useful in ion exchange chromatography and isoelectric focusing. CHAPS is as well suitable for enzyme immunoassay (6) and as a component of lysis buffer for tissue homogenization, in denaturing buffer in enzyme characterization and of 2D-sample buffer in 2D electrophoresis.
Its small micellar molecular weight and high critical micellar concentration allowing it to be removed from samples by dialysis.
CMC (25 °C) 4.2 - 6.5 mM, Na (25 °C) 9 - 10
Assay (HPLC)
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min. 97.0 %
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References:
- Hjelmeland, L.M. (1980) Proc. Natl. Acad. Sci. USA 77, 6368-70
- Naldini, L. et al. (1990) Biochemistry 29, 5153-60
- Kierdaszuk, B. & Eriksson, S. (1990) Biochemistry 29, 4109-114
- Bonfils, C. & Combalbert, J. (1990) Electrophoresis 11, 182-6
- Ambar, J. et al. (1989) Eur. J. Pharmacol. 170, 119-20
- Leblond, F.A. et al. (1989) J. Immunol. Methods 124, 71-5
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WARNING |
Hazard Statements | H315 - H319 - H335 |
Precautions | P280 |
Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P332 +P313 - P337 +P313 |
WGK: 1 • HS: 29239000
Storage Temperature: +15 °C to +30 °C