Highly purified suspension of 2x cryst. material in 70 % saturated ammonium
sulphate, pH approx. 7.2. Preparation contains predominantly isoenzymes LDH
1 and 2. LDH is used for the determination of lactate (1) and in coupled
systems for detection of biological metabolites.
Unit definition: 1 U is the amount of enzyme causing the oxidation of 1
µmole of NADH per minute at 25 °C, pH 7.4.
Extraneous activities: PK, GPT, each max. 0.01 %; MDH max. 0.05 %
Wahlefeld, A.W. (1983) Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.)
3rd Ed. Vol. 3, p. 126-33
Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, 3rd Ed., Vol. 2,
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