Dithiothreitolmolecular biology grade
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C4H10O2S2 • Mr 154.25 • CAS [3483-12-3]
Dithiothreitol, as well called DTT or Cleland's reagent, is a widely used reagent for maintaining thiol (SH) groups in reduced state. DTT quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE).
It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed.
DNase/RNase not detected. Oxidized DTT max. 0.5 %. Hygroscopic.
Assay (titr.) MP |
min. 99.0 % 40 - 45 °C |
References:
- Cleland, W.W. (1964) Biochemistry 3, 480-2
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WARNING |
Hazard Statements | H302 - H315 - H319 - H335 |
Precautions | P264 - P280 |
Reaction | P301 +P312 - P302 +P352 - P304 +P340 - P305 +P351 +P338 |
EINECS: 222-468-7 • WGK: 1 • HS: 29309098
Storage Temperature: +2 °C to +8 °C