Endoproteinase Glu-C (V8 proteinase), MS approved from Staphylococcus aureuslyophil.
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Endoproteinase Glu-C (V8) is a serine endoproteinase isolated from Staphylococcus aureus V8.
Approved quality for use with in-gel digestion and mass spectrometric analysis.
The specificity of Glu-C is primarily determined by the buffer pH and composition. Using phosphate buffers (pH 7.8), Glu-C will cleave at both glutamyl and aspartyl bonds. Ammonium bicarbonate buffer (pH 7.8) will lead to a preferential cleavage of glutamyl bonds.
The presence of proline residues on the carboxy side of the peptide bond inhibits the cleavage.
Due to its highly specific cleavage of peptides Glu-C is used in proteomics for peptide mapping and protein sequence work.
Storage Temperature: -15 °C to -25 °C