The assay bases on the bichinchoninic acid method (1). Proteins reduce
alkaline Cu(II) to Cu(I). Bichinchoninic acid forms a purple complex with
Cu(I) with an absorbance maximum at 562 nm. The absorbance is directly
proportional to protein concentration.
Fast and sensitive assay: linear detection range from 25 – 1000 µg protein/ml
Easy to use: contains ready-to-use reagents and protein standard
Compatible with many detergents
Less binding variation between different proteins than Bradford assay
How to find the required certificate of analysis for your product: In the search, enter the 5-digit article number without additional packaging, i.e. 11404 if you are looking for the AZ for your 500 g-Pack Agarose 11404.02. Now click on the search result and you will be taken to the product entry. Here you will find the link "Certificate of Analysis", which you follow. Now select the lot number from the list and download the PDF with a click. Done.
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