G 418 sulfate
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C20H40N4O10·2H2SO4 • Mr 692.7 • CAS [108321-42-2]
G 418, or Geneticin, is a aminoglycoside antibiotic from Micromonospora rhodorangea. The antibiotic is used for the selection and maintenance of eukaryotic cells expressing the neomycin resistance gene (neo). G418 blocks polypeptide synthesis by inhibiting the elongation step. It is similar in structure to gentamycin B1 but active against both bacteria and eukaryotes.
Stock solution: 10 - 50 mg/ml in H2O or cell culture medium. The working concentration has to be established for every cell type. Bacteria and algae require 5 μg/ml or less while animal cells may require 300 - 500 μg/ml.
Potency
|
min. 650 µg/mg
|
References:
- Southern, P.J. & Berg, P. (1982) J. Mol. Appl. Genetics 1, 327-41
- Bar-Nun, S. et al. (1983) Biochim. Biophys. Acta. 741, 123-7
- Hadfield, C. et al. (1990) Curr. Genet. 18, 303-13
- Wang, X. et al. (1996) Biotechnol. Bioeng. 49, 45-51
- Kunik, T. et al. (2001) PNAS 98, 1871-6
- D'Artagnan Villalba, J. et al. (2007) Microbiology 153, 3852-63
- Gietz, R.D. & Schiestl, R.H. (2007) Nature Protocols 2, 31-4
DANGER |
Hazard Statements | H317 - H334 |
Precautions | P261 - P280 |
Reaction | P302 +P352 - P304 +P340 - P333 +P313 - P342 +P311 |
Storage | P501 |
WGK: 3 • HS: 29419000
Storage Temperature: +15 °C to +30 °C