Glutamate dehydrogenase from bovine liversolution
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EC 1.4.1.3 • Mr ca. 350 000 •
ca. 40 U/mg protein
For the determination of L-glutamate, ammonia and 2-oxoglutaric acid (1, 2). Suitable for urea determination in conjunction with urease (cat. no. 37799). In 50 % glycerol.
25 mg correspond to approx. 1 ml, 100 mg correspond to approx. 4 ml.
Unit definition: 1 U causes the transformation of 1 µmole 2-oxoglutarate per minute at 25 °C and pH 7.3 under assay conditions not containing ADP.
Extraneous activities: LDH, MDH each max. 0.01 %; Ammonium ions less than 0.001 µmole per unit.
References:
- Schmidt, E. & F.W.. (1983) Methods of Enzymatic Analysis (Bergmeyer, H.U. ed.) 3rd Ed. Vol. 3, p. 216-27
- Lund, P. (1985) Methods of Enzymatic Analysis (Bergmeyer, H.U. ed.) 3rd Ed. vol. 8, p. 357-63
DANGER |
Hazard Statements | H334 |
Precautions | P261 - P281 |
Reaction | P304 +P341 - P342 +P311 |
WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C