ca. 40 U/mg protein For the determination of L-glutamate, ammonia and
2-oxoglutaric acid (1, 2). Suitable for urea determination in conjunction with
urease (cat. no. 37799). In 50 % glycerol. 25 mg correspond to approx. 1
ml, 100 mg correspond to approx. 4 ml.
Unit definition: 1 U causes the transformation of 1 µmole
2-oxoglutarate per minute at 25 °C and pH 7.3 under assay conditions not
containing ADP. Extraneous activities: LDH, MDH each max. 0.01 %;
Ammonium ions less than 0.001 µmole per unit.
Schmidt, E. & F.W.. (1983) Methods of Enzymatic Analysis (Bergmeyer, H.U. ed.)
3rd Ed. Vol. 3, p. 216-27
Lund, P. (1985) Methods of Enzymatic Analysis (Bergmeyer, H.U. ed.) 3rd Ed.
vol. 8, p. 357-63
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