Salt Active Nucleasesolution
- Pack Sizes
- Certificates of Analysis (Lot.-no. - release date)
- PDF Documents
- Additional Information
- Related Products
- Product Links
The Salt Active Nuclease completely degrades DNA and RNA in cell extracts and protein samples under high salt conditions. In the range of the activity optimum at 0.5 M NaCl, nucleic acids bound to proteins and protein complexes dissociate, whereby the endonuclease isolated from a marine bacterium also degrades DNA and RNA molecules that are inaccessible under low-salt conditions. The reaction takes place at a pH between 7 and 10 at low temperatures and in traditional buffer systems to protect the proteins.
- Non-specific endonuclease
- Optimum activity at high salt concentration (0.5 M NaCl)
- Active at low temperatures (20 % at 6 ºC)
- Broad pH range
- Easily inactivated by reducing agents
Supplied as solution in 25 mM Tris-HCl pH 7.5, 5 mM MgCl2, 0.5 M NaCl, 0.01 % Tween 20, 50 % (v/v) glycerol.
0.5 M NaCl
Storage Temperature: -15 °C to -25 °C