Salt Active Nuclease
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Supplied as solution in 25 mM Tris-HCl pH 7.5, 5 mM MgCl2, 0.5 M
NaCl, 0.01 % Triton® X-100, 50 % (v/v) glycerol.
Salt Active Nuclease is a highly active non-specific endonuclease from a marine bacterium that cleaves both DNA and RNA. It digests DNA versus RNA in a 10:1 ratio.
Salt Active Nuclease has optimum activity at 0.5 M NaCl, is active in the pH range of 7 to 10 and low temperatures, which makes the enzyme ideal for use in removal of nucleic acids from cell extracts and proteins samples. It will remove contaminating nucleic acids in a traditional protein buffer system. That guarantees the full protection of proteins while the nucleic acids are fully removed.
- Non-specific endonuclease
- Optimum activity at high salt concentration (0.5 M NaCl)
- Active at low temperatures (20 % at 6 ºC)
- Broad pH range
inactivated by reducing agents
0.5 M NaCl
Storage Temperature: -15 °C to -25 °C