LB Agar (Lennox), powder35 g for 1 liter medium
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For cultivation of E. coli in molecular biology.
10 g/l Tryptone
5 g/l Yeast extract
5 g/l NaCl
15 g/l Agar
For making 1 L liquid medium suspend 35 g in 900 ml distilled water, adjust the pH to 7.0 with approximately 0.2 ml of 5 N NaOH, fill up to a final volume of 1 L with deionized water and sterilize by autoclaving. Cool to 45 °C prior to dispensing into sterile petri dishes.
- Luria, S.E., et al., Virology 12, 348-390 (1960)
- Miller, J.H., Experiments in Molecular Genetics, p. 433, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 1972
Storage Temperature: +15 °C to +30 °C