The Proteus IMAC kit is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Proteus spin columns replace lengthy and expensive chromatographic methods such as FPLC. Metal chelate affinity chromatography is a rapid one step purification, which removes most contaminants and can achieve purities close to homogeneity.

Contents:

Quantity: 8 x 1.6 ml Ni-IMAC columns

Max. sample volume per load: 20 ml, swing bucket rotor

Collection tube: 50 ml centrifuge tubes

Min. number of purifications: 16 purifications (2 uses per column)

Typical capacity/preparation: 10 - 15 mg 6 x His-tagged protein

Vivaspin 20 ultrafiltration concentrators: None

Buffers: 5 x PBS Buffer A, 1 x Imidazole Buffer B