Recombinant Protein G Sepharose® FF Resin

Protein G Sepharose® FF Resin designed for simple, one-step and rapid antibody purification from serum, ascites and tissue culture supernatant such as those derived from static cultures and bioreactors.

Recombinant protein G has been coupled to Sepharose® to obtain a stable matrix with a high binding capacity for immunoglobulins via the heavy chain of the FC region (up to 20 mg/ml Human IgG). Antibody samples purified using this affinity resin may be used in a wide range of laboratory procedures such as 1D or 2D polyacrylamide gel electrophoresis, Western Blotting, ELISA etc. Binding affinity varies depending upon the source species and subclass.


Specificity: Protein G affinity antibodies

Matrix: Sepharose®

Coupled ligand: 2 mg Protein G/ml resin

Binding capacity (human IgG): 20 mg/ml

Bead size: 45 - 165 μm

Flow rate: 0.25 - 1 ml/min (recommended)

Maximum pressure: 120 - 140 psi

Buffer compatibility: Common aqueous buffers from pH 2.5 - 10

Binding buffer example: 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.4

Elution buffer example: 0.2 M Glycine/HCl pH 2.5

Neutralization buffer example: 1 M Tris/HCl pH 9.0

Shipping/delivery: 50 % (v/v) resin suspension in 20 % ethanol

Storage: 20 % ethanol at 2 - 8 °C for up to 2 years from manufacture

Sepharose = registered trademark of GE Healthcare

HS: 38229000

5 ml
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Electrophoresis by SERVA - Catalog 2022

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Electrophoresis by SERVA - Catalog 2022

• Isoelectric Focusing


• 2D Electrophoresis

• Native Electrophoresis

• Western Blotting

• Nucleic Acid Electrophoresis

• Equipment

SERVA Electrophoresis Catalog (PDF)
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SERVA Catalog

In detail you will find the following new products in catalog 2022:

New glycosidases such as Endo F3, Endo S and Sialidase
Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"


SERVA Catalog 2022.pdf
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