Recombinant Protein G Sepharose® FF Resin


Protein G Sepharose® FF Resin designed for simple, one-step and rapid antibody purification from serum, ascites and tissue culture supernatant such as those derived from static cultures and bioreactors.

Recombinant protein G has been coupled to Sepharose® to obtain a stable matrix with a high binding capacity for immunoglobulins via the heavy chain of the FC region (up to 20 mg/ml Human IgG). Antibody samples purified using this affinity resin may be used in a wide range of laboratory procedures such as 1D or 2D polyacrylamide gel electrophoresis, Western Blotting, ELISA etc. Binding affinity varies depending upon the source species and subclass.

Specifications

Specificity: Protein G affinity antibodies

Matrix: Sepharose®

Coupled ligand: 2 mg Protein G/ml resin

Binding capacity (human IgG): 20 mg/ml

Bead size: 45 - 165 μm

Flow rate: 0.25 - 1 ml/min (recommended)

Maximum pressure: 120 - 140 psi

Buffer compatibility: Common aqueous buffers from pH 2.5 - 10

Binding buffer example: 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.4

Elution buffer example: 0.2 M Glycine/HCl pH 2.5

Neutralization buffer example: 1 M Tris/HCl pH 9.0

Shipping/delivery: 50 % (v/v) resin suspension in 20 % ethanol

Storage: 20 % ethanol at 2 - 8 °C for up to 2 years from manufacture


Sepharose = registered trademark of GE Healthcare




HS: 38229000


Cat.No.
Size
EUR
42313.01
5 ml
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Electrophoresis by SERVA - Catalog 2022

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