Ni²⁺-IDA Metal Chelate Agarose Resin designed for affinity purification of polyhistidine tagged proteins. Nickel ions are carefully loaded onto an agarose matrix via an iminodiacetic acid (IDA) coupled ligand to obtain a stable affinity matrix with a high binding capacity for histidine residues (up to 10 mg/ml determined from E. coli cleared lysate). Other metal ions such as Co²⁺, Cu²⁺, and Zn²⁺ can also be used resulting in different affinities.
If required, the Nickel ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix recharged with a different metal ion.
Specifications
Specificity: Polyhistidine tag
Matrix: Agarose
Couples ligand: Iminodiacetic acid (IDA)
Binding capacity: 10 mg/ml
Bead size: 45 – 160 µm
Flow rate: 0.25 – 2 ml/min
Maximum pressure: 42 psi
Buffer compatibility: Common aqueous buffers from pH 2 - 12
Cleaning buffer examples: 30 % ethanol, 1 M NaOH, 0.01 M HCl, 8 M urea, 6 M guanidinium hydrochloride
Shipping/delivery: 50 % (v/v) resin suspension in 20 % ethanol at ambient temperature
Storage: Equilibration buffer at 2 - 8 °C (short-term) 20 % ethanol at 2 - 8 °C (long-term)