Ni²⁺-IDA Metal Chelate Agarose Resin designed for affinity purification of polyhistidine tagged proteins. Nickel ions are carefully loaded onto an agarose matrix via an iminodiacetic acid (IDA) coupled ligand to obtain a stable affinity matrix with a high binding capacity for histidine residues (up to 10 mg/ml determined from E. coli cleared lysate). Other metal ions such as Co²⁺, Cu²⁺, and Zn²⁺ can also be used resulting in different affinities.

If required, the nickel ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix recharged with a different metal ion.

Specifications

Specificity: Polyhistidine tag

Matrix: Agarose

Couples ligand: Iminodiacetic acid (IDA)

Binding capacity: 10 mg/ml

Bead size: 45 – 160 µm

Flow rate: 0.25 – 2 ml/min

Maximum pressure: 42 psi

Buffer compatibility: Common aqueous buffers from pH 2 - 12

Cleaning buffer examples: 30 % ethanol, 1 M NaOH, 0.01 M HCl, 8 M urea, 6 M guanidinium hydrochloride

Shipping/delivery: 50 % (v/v) resin suspension in 20 % ethanol at ambient temperature

Storage: Equilibration buffer at 2 - 8 °C (short-term) 20 % ethanol at 2 - 8 °C (long-term)