Super Nickel NTA Affinity Resin designed for affinity purification of polyhistidine tagged proteins. Nickel ions are carefully loaded onto a 7.5 % cross-linked agarose matrix (medium particle diameter 40 μm) via a NTA coupled ligand to obtain a stable affinity matrix with the highest binding capacity for histidine residues (up to 70 mg/ml determined from E. coli cleared lysate). Other metal ions such as Co²⁺, Zn²⁺, Fe³⁺, and Al³⁺ can also be used resulting in different affinities.
If required, the nickel ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix recharged with a different metal ion.
Specifications
Specificity: Polyhistidine tag
Matrix: 7.5 % cross linked agarose
Coupled ligand: Nitrilotriacetic acid (NTA)
Binding capacity: 70 mg/ml
Bead size: 32 – 60 µm (40 μm medium)
Flow rate: 0.25 – 2 ml/min (optimum), 6 ml/min (max)
Maximum pressure: 72 psi
Buffer compatibility: Common aqueous buffers from pH 2 - 14
Cleaning buffer examples: 100 % methanol, 100 % ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30 % (v/v) acetonitrile
Shipping/delivery: 50 % (v/v) resin suspension in 20 % ethanol at ambient temperature
Storage: Equilibration buffer at 2 - 8 °C (short-term) 20 % ethanol at 2 - 8 °C (long-term)