Super Nickel NTA Affinity Resin designed for affinity purification of polyhistidine tagged proteins. Nickel ions are carefully loaded onto a 7.5 % cross-linked agarose matrix (medium particle diameter 40 μm) via a NTA-coupled ligand to obtain a stable affinity matrix with the highest binding capacity for histidine residues (up to 70 mg/ml determined from E. coli cleared lysate). Other metal ions such as Co²⁺, Zn²⁺, Fe³⁺, and Al³⁺ can also be used resulting in different affinities.

If required, the Nickel ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix recharged with a different metal ion.

Specifications

Specificity: Polyhistidine tag

Matrix: 7.5 % cross linked agarose

Coupled ligand: Nitrilotriacetic acid (NTA)

Binding capacity: 70 mg/ml

Bead size: 32 – 60 µm (40 μm medium)

Flow rate: 0.25 – 2 ml/min (optimum), 6 ml/min (max)

Maximum pressure: 72 psi

Buffer compatibility: Common aqueous buffers from pH 2 - 14

Cleaning buffer examples: 100 % methanol, 100 % ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30 % (v/v) acetonitrile

Shipping/delivery: 50 % (v/v) resin suspension in 20 % ethanol at ambient temperature

Storage: Equilibration buffer at 2 - 8 °C (short-term) 20 % ethanol at 2 - 8 °C (long-term)