TEV Protease, recombinant

Mr 27 000 , 27.000

Recombinant TEV Protease is a highly site-specific cysteine protease, which is found in the Tobacco Etch Virus. Due to its sequence specificity, the enzyme is a very powerful reagent for removal of fusion tags from recombinant proteins after protein purification. The enzyme has been genetically modified to increase its activity and resistance to autolysis. It consists of the 27 kDa catalytic domain with an N-terminal polyhistidine tag.
It recognizes a seven amino acid sequence of the general form Glu-X-X-Gln-Gly/Ser, most commonly Glu-Asn-Leu-Tyr-Phe-Gln-Gly, and cleaves between glutamine and glycine or serine.
Using the polyhistidine tag at the N-terminus of the protease the enzyme can be easily removed from the cleavage reaction by affinity chromatography following digestion.
Specific activity: 1 U/µl, supplied with 1 ml 20x TEV Reaction Buffer and 100 mM DTT.



Unit definition: 1 unit cleaves >95 % of 3 µg control substrate in one hour at 30 °C.

WARNING
Hazard Statements H315 - H319
Precautions P280
Reaction P302 +P352 - P305 +P351 +P338 - P332 +P313 - P337 +P313 - P362 +P364

HS: 35079090
Storage Temperature: -15 °C to -25 °C

Cat.No.
Size
EUR
36403.01
1,000 U
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H315

Deutsch:

Verursacht Hautreizungen.

English:

Causes skin irritation.

Français:

Provoque une irritation cutanée.

Italiano:

Provoca irritazione cutanea.

Español:

Provoca irritación cutánea.

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H319

Deutsch:

Verursacht schwere Augenreizung.

English:

Causes serious eye irritation.

Français:

Provoque une sévère irritation des yeux.

Italiano:

Provoca grave irritazione oculare.

Español:

Provoca irritación ocular grave.

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P280

Deutsch:

Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

English:

Wear protective gloves/protective clothing/eye protection/face protection.

Français:

Porter des gants de protection/des vêtements de protection/un équipement de protection des yeux/du visage.

Italiano:

Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.

Español:

Llevar guantes/prendas/gafas/máscara de protección.

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P302 +P352

Deutsch:

BEI KONTAKT MIT DER HAUT: Mit viel Wasser und Seife waschen.

English:

IF ON SKIN: Wash with plenty of soap and water.

Français:

EN CAS DE CONTACT AVEC LA PEAU: laver abondamment à l’eau et au savon.

Italiano:

IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua e sapone.

Español:

EN CASO DE CONTACTO CON LA PIEL: Lavar con agua y jabón abundantes.

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P305 +P351 +P338

Deutsch:

BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

English:

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Français:

EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer

Italiano:

IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.

Español:

EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.

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P332 +P313

Deutsch:

Bei Hautreizung: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen.

English:

If skin irritation occurs: Get medical advice/attention.

Français:

En cas d’irritation cutanée: consulter un médecin.

Italiano:

In caso di irritazione della pelle: consultare un medico.

Español:

En caso de irritación cutánea: Consultar a un médico.

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P337 +P313

Deutsch:

Bei anhaltender Augenreizung: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen.

English:

If eye irritation persists: Get medical advice/attention.

Français:

Si l’irritation oculaire persiste: consulter un médecin.

Italiano:

Se l’irritazione degli occhi persiste, consultare un medico.

Español:

Si persiste la irritación ocular: Consultar a un médico.