VitalStain Blue 520for flow cytometry
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Fixable viability dyes allow accurate discrimination between live and dead cells in flow cytometric analysis, based on cell membrane integrity. The exclusion of dead cells from data allows a better identification of cell populations and provides an accurate determination of cell viability within samples.
VitalStain Blue 520 allows staining of live cells and preserve the staining pattern after fixation and/or permeabilization.
VitalStains fixable viability dyes are amine reactive and cell impermeable. Dead cells with compromised cell membranes are typically labelled to a higher extend due to reaction with intracellular amines resulting in highly fluorescent dead cells. Impermeable live cells are labelled only on the cell surface and show dim fluorescence.
VitalStain Blue 520 is excited by the blue 488 nm laser and bright
fluorescence is detected in the FITC channel.
Store for long term on -80 °C.
- High brightness for optimal differentiation between live and dead cells
- Ready-to-use kit, DMSO pre-diluted to test size formulation
- Unlike 7-AAD and PI, labelled cells can be fixed, permeabilized, washed and stained
- Suitable for any cell species
Storage Temperature: -15 °C to -25 °C