dsDNase, heat labilesolution

Mr 47 600

Unique double-strand specific endonuclease, which can be easily inactivated by heat treatment. As it does not digest ssDNA or RNA, it allows to specifically remove dsDNA in the presence of other nucleic acids. The yielding product is oligonucleotides with 5’-phosphates and 3’-hydroxyl termini.
Recombinantly produced in Pichia pastoris, specific activity ca. 200 000 units/mg.

  • Irreversible inactivation by heat treatment at 5 - 15 min at 55 ˚C, 1 mM DTT, pH ≥ 8
  • Decontamination of PCR master mixes
  • Removal of genomic DNA from RNA preparations prior to RT-qPCR



    One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2


    HS: 35079090
    Storage Temperature: -15 °C to -25 °C

  • Cat.No.
    Size
    EUR
    18545.01
    250 U
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