Peroxidase from horseradish min. 1000 U/mglyophil.

(POD, HRP, Donor: hydrogen-peroxide oxidoreductase)
EC 1.11.1.7Mr ca. 40 000CAS [9003-99-0]

For the determination of peroxide (1). Used as an indicator enzyme in reactions where peroxide is produced (2). For labelling antibodies in ELISA (3, 4). RZ (= A 403/A 275) = 3.0.

Unit definition: 1 U catalyzes the decomposition of 1 µmole hydrogen peroxide per minute at 25 °C, pH 7.0; reaction coupled with phenol- aminoantipyrine (5).
Activity in other units: min. 250 purpurogallin units/mg
(1 purpurogallin unit catalyzes the oxidation of 1 mg pyrogallol to purpurogallin in 20 seconds at 20 °C and pH 6.0. The purpurogallin is extracted and determined spectrophotometrically at 420 nm (6). 1 mg purpurogallin requires 13.5 µmole peroxide, 1 purpurogallin unit corresponds to the decomposition of 12 µmoles peroxide at 25 °C.)


References:
  1. Meiattini, F. (1985) Methods of Enzymatic Analysis, (Bergmeyer, H.U., ed.) 3rd Ed. vol. 7, p. 566-71
  2. Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, 3rd Ed. vol. 2, p. 267-8
  3. Johnson Jr., R.B. (1980) J. Immunoassay 1, 27-37
  4. Harlow & Lane (1988) Antibodies, Cold Spring Harbor Laboratory Press, p. 349
  5. Gallati, H. (1977) J. Clin. Chem. Clin. Biochem. 15, 699-703
  6. Polis, B.D. & Shmukler, H.W. (1963) J. Biol. Chem. 201, 457-500
DANGER
Hazard Statements H334
Precautions P261 - P285
Reaction P304 +P341 - P342 +P311

EINECS: 232-668-6HS: 35079090
Storage Temperature: +2 °C to +8 °C

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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.