Tris(hydroxymethyl)aminomethaneresearch grade

(TRIS, THAM, Tromethamine, Amino-2-(hydroxymethyl)-1,3-propanediol)
C4H11NO3Mr 121.1CAS [77-86-1]

Tris(hydroxymethyl)aminomethane (Tris) buffer quality for all standard applications, suitable for preparative purposes and for chromatography.
With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5. It is used as a component in buffer solutions in numerous applications:
  • Assays in molecular biology and cytology
  • Extraction of proteins or nucleic acids from cells
  • In situ hybridisation procedures
  • Sample and running buffers for SDS-PAGE
  • TAE- and TBE buffers for agarose gel electrophoresis
  • Transfer buffer for Western Blotting
  • Washing buffers for immunoassays

    Assay (titr.)
    A 1 cm/40 % in water
    290 nm
    pH (5 % in water)
    Heavy metals (Pb)

    min. 99.0 %

    max. 0.2
    10.0 - 11.5
    max. 10 ppm

    EINECS: 201-064-4WGK: 2LHS: 29221900
    Storage Temperature: +15 °C to +30 °C

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