Isoelectric Focusing
Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.
For IEF, SERVA offers a comprehensive product line:
- SERVALYT™ Carrier Ampholytes
- SERVALYT™ PRECOTES/PreNets™ Precast Gels & Kits
- FocusGels Precast Horizontal Gels
- SERVAGel™ IEF Vertical Gels
- Protein Standards
- Buffers and Accessories
- Equipment
