(Urea amidohydrolase) EC 18.104.22.168 • Mr ca. 545 000 • CAS [9002-13-5]
For the determination of urea (1).
Unit definition: 1 U catalyzes the formation of 1 µmole ammonia per
minute at 25 °C, pH 8.0 from urea, determined in a coupled reaction with GLDH
(2). Activity in other units: If the unit of activity is defined in terms of
1 µmole urea decomposed per minute, this preparation would contain at least 90
Kerscher, L. & Ziegenhorn, J. (1985) Methods of Enzymatic Analysis (Bergmeyer, H.U., ed.) 3rd Ed. Vol. 8, p. 444-53
Kaltwasser, H. & Schlegel, H.G. (1966) Anal. Biochem. 16, 132-8
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