2xYT Medium, powder31 g for 1 liter medium
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For cultivation of E. coli and M13 bacteriophages in molecular biology.
16 g/l Tryptone
10 g/l Yeast extract
5 g/l NaCl
For making 1 L 2x liquid medium suspend 31 g in 900 ml distilled water, adjust the pH to 7.0 with approximately 0.2 ml of 5 N NaOH, fill up to a final volume of 1 L with deionized water and sterilize by autoclaving.
References:
- Sambrook, J., et al., Molecular Cloning : A Laboratory Manual, 2nd ed., p. A.3, Cold Spring Harbor laboratory Press, Cold Spring Harbor, New York
HS: 38210000
Storage Temperature: +15 °C to +30 °C
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