Carbonic anhydrase from bovine erythrocytes ca. 1.5 U/mglyophil.

(Carbonate dehydratase, Carbonate hydrolyase, Karboanhydrase, Carbonsureanhydrase)
EC 4.2.1.1.Mr ca. 29 000CAS [9001-03-0]

Carbonic anhydrase preparation, which is homogeneous in SDS-PAGE. The enzyme preparation can therefore be used as a marker in protein gel electrophoresis and as a bioreagent in gel filtration chromatography, protein chromatography and plasma and blood proteins. Contains carbonic anhydrases A and B; both forms have similar high specific activities and therefore belong to the C-group of mammalian carbonic anhydrases (1).
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. It plays a main role in physiological processes like respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways.

Unit definition: 1 U catalyzes the hydrolysis of 1 µmole 4-nitrophenyl acetate per minute at 25 °C, pH 7.6 (2).
Activity in other units: ca. 4000 Wilbur-Anderson units/mg (3).


References:
  1. Lindskog, S. et al. (1971) The Enzymes (Boyer, P.D., ed.) 5, 587-665
  2. Armstrong, J. McD. et al. (1966) J. Biol. Chem. 241, 5137-49
  3. Wilbur, K.M. & Anderson, N.G. (1948) J. Biol. Chem. 176, 147-54
DANGER
Hazard Statements H334
Precautions P261 - P285
Reaction P304 +P341 - P342 +P311

EINECS: 232-576-6WGK: 1HS: 35079090
Storage Temperature: -15 °C to -25 °C

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H334

Deutsch:

Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.

English:

May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Français:

Peut provoquer des symptômes allergiques ou d'asthme ou des difficultés respiratoires par inhalation.

Italiano:

Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.

Español:

Puede provocar síntomas de alergia o asma o dificultades respiratorias en caso de inhalación.

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P261

Deutsch:

Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden.

English:

Avoid breathing dust/fume/gas/mist/vapours/spray.

Français:

Éviter de respirer les poussières/fumées/gaz/brouillards/vapeurs/aérosols.

Italiano:

Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.

Español:

Evitar respirar el polvo/el humo/el gas/la niebla/los vapores/el aerosol.

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P285

Deutsch:

Bei unzureichender Belüftung Atemschutz tragen.

English:

In case of inadequate ventilation wear respiratory protection.

Français:

Lorsque la ventilation du local est insuffisante, porter un équipement de protection respiratoire.

Italiano:

In caso di ventilazione insufficiente utilizzare un apparecchio respiratorio.

Español:

En caso de ventilación insuficiente, llevar equipo de protección respiratoria.

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P304 +P341

Deutsch:

BEI EINATMEN: Bei Atembeschwerden an die frische Luft bringen und in einer Position ruhigstellen, die das Atmen erleichtert.

English:

IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

Français:

EN CAS D’INHALATION: s'il y a difficulté à respirer, transporter la victime à l'extérieur et la maintenir au repos dans une position où elle peut confortablement respirer.

Italiano:

IN CASO DI INALAZIONE: se la respirazione è difficile, trasportare l'infortunato all’aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.

Español:

EN CASO DE INHALACIÓN: Si respira con dificultad, transportar a la víctima al exterior y mantenerla en reposo en una posición confortable para respirar.

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P342 +P311

Deutsch:

Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.

English:

If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician.

Français:

En cas de symptômes respiratoires: appeler un CENTRE ANTIPOISON ou un médecin.

Italiano:

In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI o un medico.

Español:

En caso de síntomas respiratorios: Llamar a un CENTRO DE INFORMACIÓN TOXICOLÓGICA o a un médico.