Tween® 80pure, Ph. Eur., USP/NF

(Polysorbate 80, Polyoxyethylene sorbitan monooleate)
Mr ca. 1300CAS [9005-65-6]

Tween® 80, belonging to the family of polysorbates, is non-ionic surfactant for extraction of membrane proteins and isolation of cell nuclei from mammalian cell lines. The detergent is used as a carrier solution for specific injections in mice and rats and for the determination of esterase activity in bacterial cells. Industrially, Tween® 80 is used as a stabiliser and emulsifier.
Tested for use in tissue culture. n ca. 20, CMC (25 °C) 0.01 mM, HLB 15.0

Density (25 °C)
Ethylene oxide value
1,4-Dioxane content
Heavy metals
Non animal origin
1.06 - 1.09
max. 1 ppm
max. 10 ppm
max. 10 ppm
Tween 80 = registered trademark of CRODA International PLC

  1. Sato, M. et al. (1989) Int. J. Biochem. 21, 751-4
  2. Masaki, S. et al. (1990) Microbiol. Immunol. 34, 653-63
  3. Okuno, S. & Fujisawa, H. (1990) Biochim. Biophys. Acta 1038, 204-8

EINECS: 500-019-9WGK: 1LHS: 34024200
Storage Temperature: +15 °C to +30 °C

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