A multi-component enzyme system (1). Although the preparation has high
cellulase activity, it still contains hemicellulases, and it degrades mannans,
xylans, galactomannans, pectins and other polysaccharides. Widely used for the
isolation of protoplasts, for its ability to degrade cell walls, often in
combination with Macerozyme R-10 (cat. no. 28302) (2). Temperature
optimum: 40 - 50 °C pH-optimum: pH 4 - 5
Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from
sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined
with alkaline copper reagent (3). Extraneous activities: α-amylase ca. 0.8 U, pectinase ca. 0.4 U,
protease ca. 0.01 DMC-U, hemicellulase ca. 1 U/mg (1 U catalyzes the
liberation of 1 µmole reducing groups from xylan per hour at 37 °C, pH 5.5,
calculated as xylose).
Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.
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