A multi-component enzyme system (1). Although the preparation has high
cellulase activity (EC 126.96.36.199), it still contains hemicellulases, and it
degrades mannans, xylans, galactomannans, pectins and other polysaccharides.
Contains about three times as high xylanase activity as Cellulase Onozuka R-10
(cat. no. 16419). Widely used for the isolation of protoplasts, for its
ability to degrade cell walls, often in combination with Macerozyme R-10 (cat.
no. 28302) (2). Temperature optimum: 50 - 60 °C pH-optimum:
pH 4 - 5
Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from
sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined
with alkaline copper reagent (3). Extraneous activities: Contains α-amylase, pectinase, protease and
Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.
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